QIAGEN Multiplex PCR Kit (1000) contains 1 x Multiplex PCR MasterMix 2x, (1 x 25 ml) (Mastermix contains HotStarTaq® DNA Polymerase, Multiplex PCR Buffer*, dNTP Mix) 1 x Q-Solution (10ml) 1 x RNase-free water (20 ml) Handbook