QIAGEN LongRange 2Step RT-PCR Kit (100) For 100 PCR rxns a 50 µl: cDNA Step, 50 RT rxns a 20 µl : LongRange RT enzyme, LongRange RT reaction buffer, dNTP´s, Oligo dT, RNAse free water PCR Step: QIAGEN LongRange PCR Kit (100)