QIAGEN LongRange 2Step RT-PCR Kit (20) For 20 PCR rxns a 50 µl: cDNA Step, 10 RT rxns a 20 µl: LongRange RT enzyme, LongRange RT reaction buffer, dNTP´s, Oligo dT, RNAse free water PCR Step: QIAGEN LongRange PCR Kit (20)